Fig. 4
From: AlphaFold 3 sheds insights into chemical enhancer-induced structural changes in Cas12a RNPs
Structural analysis of activated Cas12a RNP with BSA on the basis of AlphaFold3. A Structural comparison between simulation models based on AlphaFold3 via the activated Cas12a RNP (purple) and the BSA-enhanced activated Cas12a RNP (palegreen) (RMSD = 0.384). B Molecular simulation estimates of distance changes between Val377 and Gln1136 due to the Cas12a conformation changes resulting from BSA by AlphaFold3. The Val377–Gln1136 distance in activated Cas12a RNP with BSA is 33.19 Å, which is similar to the 32.55 Å distance in activated Cas12a RNP. Green: Cas12a protein; blue: nuclease domain (NUC) in Cas12a; parakeet: gRNA; slate: ssDNA trigger; brown: BSA