Fig. 2

a–d, Comparison between Antares–CFz and other luciferase reporters in transgenic mice. a, Representative images of peak bioluminescence of reporters expressed in VGAT+ neurons. b, Bioluminescence intensity over time. Data are presented as mean values ± s.e.m.; n = 3 independent animals in each condition. c, Representative images of peak bioluminescence of reporters expressed in CaMKIIα+ neurons. d, Bioluminescence intensity over time. Data are presented as mean values ± s.e.m.; n = 7, 4, 3, 4 and 4 animals for AkaLuc with 3 µmol of AkaLumine, AkaLuc with 1.3 µmol of AkaLumine, Antares with 1.3 µmol of CFz, FLuc with 0.62 µmol of CycLuc or FLuc with 13 µmol of D-luciferin, respectively. e, Representative bioluminescence images of Antares–CFz and AkaLuc–AkaLumine in the hippocampus of J:NU mice co-infected with Antares- and AkaLuc-encoded AAV. f, Comparison of peak signal intensities. Data are presented as mean values ± s.e.m.; n = 4 animals, each of which was measured for both Antares–CFz and AkaLuc–AkaLumine signals. P values were determined by two-tailed Student’s paired t-test.Copyright ref [44], 2023 Nature Publishing Group