Fig. 6
From: Optical imaging in biomedical research: guidelines and practical insights
Circulatory in vivo half-life of the FLECT fluoroprobe and its use in FLECT/CT imaging of mice with left carotid ferric chloride induced thrombus. A Mice (n=5) were i.v. injected with 1 µg/g of Targ-Cy7 and blood was collected at different time-points (0, 5, 30, 60 120, 240 and 1440 minutes). The NIR fluorescence signal in the collected samples was determined by the IVIS® Lumina imager and quantified as shown. B Upon arterial thrombus formation using the ferric chloride model, mice were i.v. injected with either mutated (Mut-Cy7; top panel) or targeting-fluoroprobe (Targ-Cy7; bottom panel) and allowed to circulate before they were scanned on the FLECT/CT imager. Following data reconstruction, coregistration and analysis, a representative comparison of maximum-intensity projection of FLECT/CT images of Mut-Cy7 (n=6) and Targ-Cy7 (n=6) mice is shown. The colour scale for each FLECT/CT image shows levels of detected NIR fluorescence with white corresponding to the highest intensity and blue the lowest. C Using Invivoscope software, the region of interest around the left carotid artery was determined, and detected fluorescence intensity was quantified between groups of mice (**: p ≤ 0.01; Mann-Whitney nonparametric test, p= 0.0022). D A representative micrograph of the ferric chloride-injured carotid artery (top) and the contralateral uninjured carotid artery (bottom), where nuclear stain (DAPI) is blue, and platelet-specific (CD41- Allophycocyanin) is red. E Further analysis of the detected FLECT-signal in each mouse shows a strongly significant correlation to the weight of its ex-vivo thrombus (using Pearson's correlation analysis: r = 0.9807 and p= 0.0006, ***). Copyright ref [117], Ivy Spring 2017