Table 1 A simplified example of the influence of background subtraction on exaggerating fold differences in low signal measurements
From: Optical imaging in biomedical research: guidelines and practical insights
Average Bck | Average Gr1 | Average Gr2 | Ratio Gr2/Gr1 | Â |
|---|---|---|---|---|
1000 | 1100 | 1200 | Â | Â |
(Gr – Bck) | 100 (1100–1000) | 200 (1200–1000) | twofold 200/100 | subtraction |
(Gr/Bck) | 1.1 (1100/1000) | 1.2 (1200/1000) | 1.09-fold 1.2/1.1 | division |